HAEM-LOKᵀᴹ Collection Tube for Cell-Free DNA
A 21G or larger diameter needle is recommended to minimize hemolysis. Either a BD “butterfly needle” (BD Cat. No. 367281 Blood Collection Set with Tubing) or standard safety tube holder may be used.
It is recommended that the HAEM-Lok™ Tube be drawn after an EDTA tube.
The total fill volume is approximately 9.0 mL.
As soon as possible, preferably within 2 min. Invert gently after collection to mix the additive with the blood. Please refer to the Instructional Video.
Yes. The matrix is not soluble. It will remain at the bottom of the tube until after centrifugation.
The HAEM-Lok™ Tube should be held at room temperature for no longer than 2 hr after blood collection and prior to centrifugation. Alternatively, the filled Tube may be held at 4C for up to 3 days after blood collection and prior to centrifugation.
A variable degree of cell settling occurs prior to centrifugation. Mixing redistributes the cells back to the optimal configuration to remove them during the centrifugation step.
The HAEM-Lok™ Tube can be centrifuged in a swinging-bucket rotor at 1600 – 3000g or in a fixed-angle rotor at 1100g with similar results.
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